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  4. Recombinant Escherichia coli Lipid A palmitoyltransferase PagP (pagP)

Recombinant Escherichia coli Lipid A palmitoyltransferase PagP (pagP)

  • 中文名稱：
    大腸桿菌pagP重組蛋白
  • 貨號：
    CSB-YP334212ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌pagP重組蛋白
  • 貨號：
    CSB-EP334212ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌pagP重組蛋白
  • 貨號：
    CSB-EP334212ENV-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌pagP重組蛋白
  • 貨號：
    CSB-BP334212ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌pagP重組蛋白
  • 貨號：
    CSB-MP334212ENV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pagP
  • Uniprot No.：
  • 別名：
    pagP; crcA; ybeG; b0622; JW0617; Lipid A palmitoyltransferase PagP; EC 2.3.1.251; Lipid A acylation protein
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    26-186
  • 氨基酸序列
    NADEW MTTFRENIAQ TWQQPEHYDL YIPAITWHAR FAYDKEKTDR YNERPWGGGF GLSRWDEKGN WHGLYAMAFK DSWNKWEPIA GYGWESTWRP LADENFHLGL GFTAGVTARD NWNYIPLPVL LPLASVGYGP VTFQMTYIPG TYNNGNVYFA WMRFQF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transfers a palmitate residue from the sn-1 position of a phospholipid to the N-linked hydroxymyristate on the proximal unit of lipid A or its precursors. Phosphatidylglycerol (PtdGro), phosphatidylethanolamine (PtdEtn), phosphatidylserine (PtdSer) and phosphatidic acid (Ptd-OH) are all effective acyl donors.
  • 基因功能參考文獻：
    1. Data suggest that, as PagP folds and interacts with lipid bilayer/micelle, thermodynamic contribution of interface amino acid residues correlates with change in non-polar accessible surface area; C-terminal beta-sheet motif is a recognition signal and it exerts considerable influence on folding cooperativity and stability of the folded beta-barrel. PMID: 28592485
    2. the effects of changing the membrane composition and the relative concentrations of protein and lipid on the folding mechanism of the bacterial outer membrane protein PagP were investigated. PMID: 22245579
    3. Molecular dynamics, mutagenesis, and a 1.4 A crystal structure of PagP in an SDS / 2-methyl-2,4-pentanediol cosolvent system reveal that phospholipid access occurs at the crenel present between strands F and G of PagP. PMID: 20826347
    4. Within the hydrophobic and van der Waals-contacted environment of the PagP hydrocarbon ruler, side chain flexibility, combined with localized thioether-aromatic dispersion attraction, likely influences the specificity of acyl chain selection. PMID: 20853818
    5. crystal structure; a striking structural similarity between PagP and the lipocalins suggests an evolutionary link between these proteins PMID: 15272304

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  • 亞細胞定位：
    Cell outer membrane.
  • 蛋白家族：
    Lipid A palmitoyltransferase family
  • 數(shù)據(jù)庫鏈接：


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